Device

Part:BBa_J64033

Designed by: Dan Widmaier   Group: Voigt Lab   (2008-01-16)


pCASP - Chaperone

This is a variant of the pCASP secretion circuit lacking the chaperone (sicP) for the SptP167 secretion tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1388
    Illegal PstI site found at 779
    Illegal PstI site found at 1009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 779
    Illegal PstI site found at 1009
    Illegal NotI site found at 1305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1388
    Illegal PstI site found at 779
    Illegal PstI site found at 1009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1388
    Illegal PstI site found at 779
    Illegal PstI site found at 1009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1253


[edit]
Categories
Parameters
None